The Department of Biology at the Syed Babar Ali School of Science and Engineering (SBASSE) presents
"Engineered Saccharomyces Cerevisiae for β-amyrin Production and Transporter’s Screening for its Extracellular Efflux"
Date: Wednesday, September 19, 2018
Time: 11:00 am – 12:00 pm
Venue: SBASSE Dean’s Smart Lab, 4th floor, SBASSE Building, LUMS
Guest Speaker: Dr. Muhammad Saad Ahmed
Host: Dr. Amir Faisal
Brief Biography
Dr. Muhammad Saad Ahmed has completed his PhD in Biochemical Engineering, with a specialisation and research focused on Metabolic Engineering and Synthetic Biology from the School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China. Moreover, Dr. Ahmed had good publications in world prestigious and high-impact factor journals, i.e. Nucleic Acids Research, Chemical Engineering Science, Chemical and Biochemical Engineering, Applied Microbiology and Biotechnology, Molecular Neurobiology, and certain other Journals.
Abstract
Metabolic Engineering and Synthetic Biology strategies in Saccharomyces cerevisiae were used to enhance the β-amyrin production. Key enzymes of the mevalonate pathway were overexpressed that enhanced the production of β-amyrin up to 10.86 mg/L. A set of 23 short (33-66 bp) synthetic terminators were designed, characterised, and analysed. The expression enhancing synthetic terminators were employed downstream of the key enzymes that aggrandised 3.14-fold production of β-amyrin. Finally, using 5L fermenter the production of β-amyrin was achieved up to 108.26 mg/L along with 38.8 mg/L of ergosterol.
